Hotspot

ndhA-ndhA

Genome · 124785-125852 · 1068 bp

Pi 0.000943

MarkerSeek score 60.00

Primer available yes

Label ndhA-ndhA

Feature Metadata

label_name: ndhA-ndhA
feature_type: igs
region: Genome
length_bp: 1068
variable_sites: 1
pi: 0.000943
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA-ndhA_p1	CACAGATTCCACCCCCAAAA	TTAGGTGGTCTACGAGCTGC	50.0	55.0	58.0	59.2	1213-1220 bp	1.0	71.1
ndhA-ndhA_p2	AGATTCCACCCCCAAAAACC	TTAGGTGGTCTACGAGCTGC	50.0	55.0	58.0	59.2	1210-1217 bp	1.0	70.9
ndhA-ndhA_p3	CACAGATTCCACCCCCAAAA	TTTAGGTGGTCTACGAGCTG	50.0	50.0	58.0	56.7	1214-1221 bp	1.0	70.0
ndhA-ndhA_p4	AGATTCCACCCCCAAAAACC	TTTAGGTGGTCTACGAGCTG	50.0	50.0	58.0	56.7	1211-1218 bp	1.0	69.8
ndhA-ndhA_p5	GATTCCACCCCCAAAAACCA	TTAGGTGGTCTACGAGCTGC	50.0	55.0	58.3	59.2	1209-1216 bp	1.0	68.9