Hotspot

rrn16-trnI

Genome · 106076-106391 · 316 bp

Back to Ananas

Pi 0.0443
MarkerSeek score 78.30
Primer available yes
Label rrn16-trnI

Feature Metadata

label_name
rrn16-trnI
feature_type
igs
region
Genome
length_bp
316
variable_sites
14
pi
0.0443
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn16-trnI_p1 CAAGGTAGCCGTACTGGAAG GGCACAACGACGCAATTATC 55.0 50.0 57.7 58.2 410 bp 1.0 78.9
rrn16-trnI_p2 CAAGGTAGCCGTACTGGAAG CTATCCATGTGGCTGAGAGC 55.0 55.0 57.7 57.8 442 bp 1.0 78.8
rrn16-trnI_p3 CAAGGTAGCCGTACTGGAAG CGCTGATGAGCACATTGAAC 55.0 50.0 57.7 57.8 461 bp 1.0 78.7
rrn16-trnI_p4 AGTGACTGGAGTGAAGTCGT CTATCCATGTGGCTGAGAGC 50.0 55.0 58.3 57.8 464 bp 1.0 78.6
rrn16-trnI_p5 AGTGACTGGAGTGAAGTCGT CGCTGATGAGCACATTGAAC 50.0 50.0 58.3 57.8 483 bp 1.0 78.5