Hotspot

trnW-trnP

Genome · 69726-69872 · 147 bp

Back to Ananas

Pi 0.00704
MarkerSeek score 59.80
Primer available yes
Label trnW-trnP

Feature Metadata

label_name
trnW-trnP
feature_type
igs
region
Genome
length_bp
147
variable_sites
1
pi
0.00704
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-trnP_p1 GCGTTCTACCGAACTGAACT GTCACGGGTTCAAATCCTGT 50.0 50.0 57.9 58.1 196-201 bp 1.0 81.1
trnW-trnP_p2 GCGTTCTACCGAACTGAACT CACGGGTTCAAATCCTGTCA 50.0 50.0 57.9 58.1 194-199 bp 1.0 81.1
trnW-trnP_p3 GCGTTCTACCGAACTGAACT GGGTACAAAATGTCACGGGT 50.0 50.0 57.9 58.1 207-212 bp 1.0 81.0
trnW-trnP_p4 GCGTTCTACCGAACTGAACT TGGGTACAAAATGTCACGGG 50.0 50.0 57.9 58.1 208-213 bp 1.0 81.0
trnW-trnP_p5 GCGTTCTACCGAACTGAACT TGTCACGGGTTCAAATCCTG 50.0 50.0 57.9 58.1 197-202 bp 1.0 81.0