Hotspot

petA-psbJ

Genome · 66020-66821 · 802 bp

Back to Ananas

Pi 0.00748
MarkerSeek score 63.10
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
Genome
length_bp
802
variable_sites
6
pi
0.00748
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCCTTTTGTTCTTCTTCGC TCTGGGTTGGGTTCATCTCT 50.0 50.0 57.9 58.0 928-1240 bp 1.0 79.7
petA-psbJ_p2 GGCCTTTTGTTCTTCTTCGC TGGGTTGGGTTCATCTCTCT 50.0 50.0 57.9 58.0 926-1238 bp 1.0 79.7
petA-psbJ_p3 GGCCTTTTGTTCTTCTTCGC CTGGGTTGGGTTCATCTCTC 50.0 55.0 57.9 57.6 927-1239 bp 1.0 76.7
petA-psbJ_p4 CGTCCAAGGCCTTTTGTTCT TCTGGGTTGGGTTCATCTCT 50.0 50.0 58.7 58.0 935-1247 bp 1.0 74.6
petA-psbJ_p5 CGTCCAAGGCCTTTTGTTCT TGGGTTGGGTTCATCTCTCT 50.0 50.0 58.7 58.0 933-1245 bp 1.0 74.6