Hotspot

accD-psaI

Genome · 61356-62341 · 986 bp

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Pi 0.00306
MarkerSeek score 61.10
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
Genome
length_bp
986
variable_sites
3
pi
0.00306
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 AGCTCCATGGTTTCTTTCCC CCATTGCAATTGCCGGAAAT 50.0 45.0 57.8 58.0 1094-1100 bp 1.0 78.7
accD-psaI_p2 GCTCCATGGTTTCTTTCCCT CCATTGCAATTGCCGGAAAT 50.0 45.0 57.8 58.0 1093-1099 bp 1.0 78.7
accD-psaI_p3 AGCTCCATGGTTTCTTTCCC AAGAAGCCATTGCAATTGCC 50.0 45.0 57.8 57.9 1100-1106 bp 1.0 78.0
accD-psaI_p4 GCTCCATGGTTTCTTTCCCT AAGAAGCCATTGCAATTGCC 50.0 45.0 57.8 57.9 1099-1105 bp 1.0 78.0
accD-psaI_p5 GACCCAATCGTACCACGTAA CCATTGCAATTGCCGGAAAT 50.0 45.0 57.3 58.0 1146-1152 bp 1.0 75.2