Hotspot

rps16

LSC · 5214-6320 · 1107 bp

Back to Anacyclus

Pi 0.00431

MarkerSeek score 62.40

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1107
variable_sites: 7
pi: 0.00431
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGAAATCATTATCCTACCCTTAT	TCTCTGTACCGACTGAACCA	30.4	50.0	52.1	57.7	276-1254 bp	1.0	44.5
rps16_p2	ATAGAAATCATTATCCTACCCTT	TCTCTGTACCGACTGAACCA	30.4	50.0	52.1	57.7	278-1256 bp	1.0	44.5
rps16_p3	AGAAATCATTATCCTACCCTTATT	TCTCTGTACCGACTGAACCA	29.2	50.0	53.0	57.7	276-1254 bp	1.0	44.5
rps16_p4	TGATATAGAAATCATTATCCTACCCT	TCTCTGTACCGACTGAACCA	30.8	50.0	54.9	57.7	282-1260 bp	1.0	44.5
rps16_p5	AGAAATCATTATCCTACCCTTATTT	TCTCTGTACCGACTGAACCA	28.0	50.0	53.8	57.7	276-1254 bp	1.0	44.5