Hotspot

rpl32-trnL-UAG

SSC · 111797-112698 · 902 bp

Back to Anacyclus

Pi 0.00462

MarkerSeek score 52.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 902
variable_sites: 9
pi: 0.00462
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGTACGACAAACAAATAAGTCCT	AGCGTGTCTACCGATTTCAC	34.8	50.0	56.2	58.0	956-999 bp	1.0	44.6
rpl32-trnL-UAG_p2	TGTACGACAAACAAATAAGTCCT	CAGCGTGTCTACCGATTTCA	34.8	50.0	56.2	58.0	957-1000 bp	1.0	44.5
rpl32-trnL-UAG_p3	TGTACGACAAACAAATAAGTCCT	TCCTAAGAGCAGCGTGTCTA	34.8	50.0	56.2	57.9	966-1009 bp	1.0	43.6
rpl32-trnL-UAG_p4	TGTACGACAAACAAATAAGTCCT	CCTAAGAGCAGCGTGTCTAC	34.8	55.0	56.2	57.8	965-1008 bp	1.0	43.3
rpl32-trnL-UAG_p5	TGTACGACAAACAAATAAGTCCT	CTAAGAGCAGCGTGTCTACC	34.8	55.0	56.2	57.8	964-1007 bp	1.0	43.3