Hotspot

rpl14-rpl16

LSC · 80701-80822 · 122 bp

Back to Anacyclus

Pi 0.0124

MarkerSeek score 60.90

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 2
pi: 0.0124
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	ACACATCAATTCTCGAGCCC	AATAGCAGCGTCCAAAATGC	50.0	45.0	58.0	57.4	233-326 bp	1.0	77.1
rpl14-rpl16_p2	ACACATCAATTCTCGAGCCC	GCGTCCAAAATGCCTATACG	50.0	50.0	58.0	57.3	226-319 bp	1.0	76.4
rpl14-rpl16_p3	TTGCTCGCTCCTATGATTCG	AATAGCAGCGTCCAAAATGC	50.0	45.0	57.9	57.4	256-349 bp	1.0	76.1
rpl14-rpl16_p4	TTGCTCGCTCCTATGATTCG	GCGTCCAAAATGCCTATACG	50.0	50.0	57.9	57.3	249-342 bp	1.0	75.4
rpl14-rpl16_p5	CGATTGCTCGCTCCTATGAT	AATAGCAGCGTCCAAAATGC	50.0	45.0	57.6	57.4	259-352 bp	1.0	74.3