Hotspot

petA-psbJ

LSC · 62984-63746 · 763 bp

Back to Anacyclus

Pi 0.000528

MarkerSeek score 59.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 763
variable_sites: 1
pi: 0.000528
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCC	50.0	50.0	56.8	57.6	823-845 bp	1.0	68.5
petA-psbJ_p2	CGCATCTGTTATTTTGGCACA	ATTCCGGATTGGGTTCATCC	42.9	50.0	57.8	57.6	874-896 bp	1.0	68.0
petA-psbJ_p3	CGAGAAGGTTCAATTGTCCGA	ATTCCGGATTGGGTTCATCC	47.6	50.0	58.3	57.6	823-845 bp	1.0	67.2
petA-psbJ_p4	TCGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCC	47.6	50.0	58.3	57.6	824-846 bp	1.0	67.2
petA-psbJ_p5	CGAGAAGGTTCAATTGTCCG	TGTGATCGGTTTAGTAGGCG	50.0	50.0	56.8	57.4	861-883 bp	1.0	66.8