Hotspot

ndhC-trnV-UAC

LSC · 49855-52219 · 2365 bp

Back to Anacyclus

Pi 0.00881

MarkerSeek score 55.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 2365
variable_sites: 23
pi: 0.00881
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.45
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCCTTTGCTACTCGGAGCTA	TTAAGTGCCAAACAGACCCC	50.0	50.0	57.9	58.0	1638-2949 bp	1.0	83.6
ndhC-trnV-UAC_p2	GCGTCGCCTATTGGTTCTAT	TTAAGTGCCAAACAGACCCC	50.0	50.0	57.8	58.0	1688-2999 bp	1.0	83.5
ndhC-trnV-UAC_p3	ACTCGGAGCTAAAAATCCCG	TTAAGTGCCAAACAGACCCC	50.0	50.0	57.7	58.0	1629-2940 bp	1.0	82.3
ndhC-trnV-UAC_p4	TTCTCCGGTCCTTTGCTACT	TTAAGTGCCAAACAGACCCC	50.0	50.0	58.4	58.0	1646-2957 bp	1.0	81.9
ndhC-trnV-UAC_p5	TCCTTTGCTACTCGGAGCTA	CCAAACAGACCCCCATCATT	50.0	50.0	57.9	57.8	1631-2942 bp	1.0	81.8