Hotspot

pafI-trnS-GGA

LSC · 43771-44612 · 842 bp

Back to Anacyclus

Pi 0.00178

MarkerSeek score 59.90

Primer available yes

Label pafI-trnS-GGA

Feature Metadata

label_name: pafI-trnS-GGA
feature_type: igs
region: LSC
length_bp: 842
variable_sites: 3
pi: 0.00178
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafI-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTCAA	45.0	45.0	56.4	57.8	977-998 bp	1.0	66.0
pafI-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	TTCCAATGCTACGCCTTCAA	45.0	45.0	56.4	57.8	978-999 bp	1.0	66.0
pafI-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	GGATTCGAACCCTCGGTAAG	45.0	55.0	56.4	57.8	1015-1036 bp	1.0	65.8
pafI-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	GGATTCGAACCCTCGGTAAG	45.0	55.0	56.4	57.8	1016-1037 bp	1.0	65.8
pafI-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	AGTTCCAATGCTACGCCTTC	45.0	50.0	56.4	58.3	979-1000 bp	1.0	65.5