Hotspot

trnG-UCC-psbD

LSC · 30751-32183 · 1433 bp

Back to Anacyclus

Pi 0.00216

MarkerSeek score 60.20

Primer available yes

Label trnG-UCC-psbD

Feature Metadata

label_name: trnG-UCC-psbD
feature_type: igs
region: LSC
length_bp: 1433
variable_sites: 7
pi: 0.00216
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-psbD_p1	AACCGAAAGACCCCTTAACT	GAAACGGTCCCTACGTAACC	45.0	55.0	56.4	58.0	1589-1633 bp	1.0	67.3
trnG-UCC-psbD_p2	AACCGAAAGACCCCTTAACT	GGACCAGCCTACAAAAACGA	45.0	50.0	56.4	58.1	1607-1651 bp	1.0	66.4
trnG-UCC-psbD_p3	AAACCGAAAGACCCCTTAACT	GAAACGGTCCCTACGTAACC	42.9	55.0	57.1	58.0	1590-1634 bp	1.0	65.5
trnG-UCC-psbD_p4	AACCGAAAGACCCCTTAACT	ACAAAAACGAAACGGTCCCT	45.0	45.0	56.4	58.2	1597-1641 bp	1.0	65.4
trnG-UCC-psbD_p5	AAACCGAAAGACCCCTTAACT	GGACCAGCCTACAAAAACGA	42.9	50.0	57.1	58.1	1608-1652 bp	1.0	64.6