Hotspot

psbA-trnK-UUU

LSC · 1522-1742 · 221 bp

Back to Anacyclus

Pi 0.0187

MarkerSeek score 68.40

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 221
variable_sites: 6
pi: 0.0187
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	AAGACGGTTTTCGGTGCTAG	GAAAGCCGTGTGCAATGAAA	50.0	45.0	58.2	57.9	392-422 bp	1.0	82.4
psbA-trnK-UUU_p2	AAAGACGGTTTTCGGTGCTA	GAAAGCCGTGTGCAATGAAA	45.0	45.0	57.8	57.9	393-423 bp	1.0	82.2
psbA-trnK-UUU_p3	TAAAGACGGTTTTCGGTGCT	GAAAGCCGTGTGCAATGAAA	45.0	45.0	57.8	57.9	394-424 bp	1.0	82.2
psbA-trnK-UUU_p4	AAGACGGTTTTCGGTGCTAG	CTAGTTCCGGGTTCGAATCC	50.0	55.0	58.2	57.8	307-337 bp	1.0	82.0
psbA-trnK-UUU_p5	GTAAAGACGGTTTTCGGTGC	GAAAGCCGTGTGCAATGAAA	50.0	45.0	57.7	57.9	395-425 bp	1.0	81.7