Hotspot

ndhH

SSC · 118055-118670 · 616 bp

Back to Anacolosa

Pi 0.0579

MarkerSeek score 75.40

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 616
variable_sites: 35
pi: 0.0579
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GAGATTCCAACCGCTCAAGT	TGGAAAACGTCAACGACTGA	50.0	45.0	57.9	57.7	1137-1939 bp	1.0	80.1
ndhH_p2	GAGATTCCAACCGCTCAAGT	AATCAAGCGGGGAAAAACCT	50.0	45.0	57.9	58.0	951 bp	0.5	59.1
ndhH_p3	GAGATTCCAACCGCTCAAGT	TACACGCATTGTCGTCCATT	50.0	45.0	57.9	57.9	1555 bp	0.5	58.5
ndhH_p4	GAGATTCCAACCGCTCAAGT	GGAAGAATCAAGCGGGGAAA	50.0	50.0	57.9	58.2	956 bp	0.5	57.9
ndhH_p5	GAGAGATTCCAACCGCTCAA	AATCAAGCGGGGAAAAACCT	50.0	45.0	57.6	58.0	953 bp	0.5	56.8