Hotspot

trnN-GUU-trnR-ACG

SSC · 125429-125740 · 312 bp

Back to Anacolosa

Pi 0.0737

MarkerSeek score 70.10

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: SSC
length_bp: 312
variable_sites: 23
pi: 0.0737
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TACCCTTGTCTCGGTTCCTT	GCGGAAAAATAGCTCGATGC	50.0	50.0	58.0	58.0	1252 bp	0.5	59.6
trnN-GUU-trnR-ACG_p2	TACCCTTGTCTCGGTTCCTT	CCTTGTTCCTACATGACCCG	50.0	55.0	58.0	58.0	1554 bp	0.5	59.6
trnN-GUU-trnR-ACG_p3	TACCCTTGTCTCGGTTCCTT	TGTCCCCTCCAGTCAAGAAT	50.0	50.0	58.0	58.0	1430 bp	0.5	59.5
trnN-GUU-trnR-ACG_p4	TACCCTTGTCTCGGTTCCTT	GTCAAGTGGAAGTGCAGTGA	50.0	50.0	58.0	58.0	1615 bp	0.5	59.5
trnN-GUU-trnR-ACG_p5	TACCCTTGTCTCGGTTCCTT	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	1589 bp	0.5	59.4