Hotspot

psaC-ndhI

SSC · 116761-117295 · 535 bp

Back to Anacolosa

Pi 0.0515

MarkerSeek score 76.00

Primer available yes

Label psaC-ndhI

Feature Metadata

label_name: psaC-ndhI
feature_type: igs
region: SSC
length_bp: 535
variable_sites: 27
pi: 0.0515
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhI_p1	ATACATCTGTAGGGCAGGCT	ACCTGTAACTATTTAATATCCTTATCA	50.0	25.9	57.9	54.1	643-656 bp	1.0	44.7
psaC-ndhI_p2	ATACATCTGTAGGGCAGGCT	TCTTTACCTGTAACTATTTAATATCCT	50.0	25.9	57.9	54.0	648-661 bp	1.0	44.6
psaC-ndhI_p3	TGCTTTACATCCGTCCCAAG	ACCTGTAACTATTTAATATCCTTATCA	50.0	25.9	57.9	54.1	675-688 bp	1.0	44.5
psaC-ndhI_p4	ACATCCGTCCCAAGGTATCA	ACCTGTAACTATTTAATATCCTTATCA	50.0	25.9	58.1	54.1	669-682 bp	1.0	44.5
psaC-ndhI_p5	TGCTTTACATCCGTCCCAAG	TCTTTACCTGTAACTATTTAATATCCT	50.0	25.9	57.9	54.0	680-693 bp	1.0	44.4