Hotspot

ndhD-psaC

SSC · 116312-116514 · 203 bp

Back to Anacolosa

Pi 0.0543

MarkerSeek score 69.80

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 203
variable_sites: 10
pi: 0.0543
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AGGATACATAGGCTGTTAAAAGT	AGCATGGGTCTAGCTTATTGA	34.8	42.9	55.1	56.5	243-248 bp	1.0	47.6
ndhD-psaC_p2	GGATACATAGGCTGTTAAAAGT	AGCATGGGTCTAGCTTATTGA	36.4	42.9	53.6	56.5	242-247 bp	1.0	47.6
ndhD-psaC_p3	AGGATACATAGGCTGTTAAAAGT	GCATGGGTCTAGCTTATTGA	34.8	45.0	55.1	54.9	242-247 bp	1.0	47.6
ndhD-psaC_p4	AGGATACATAGGCTGTTAAAAGT	AGCATGGGTCTAGCTTATTG	34.8	45.0	55.1	54.9	243-248 bp	1.0	47.6
ndhD-psaC_p5	AGGATACATAGGCTGTTAAAAGTT	AGCATGGGTCTAGCTTATTGA	33.3	42.9	55.8	56.5	248 bp	0.5	20.0