Hotspot

trnL-UAG-ccsA

SSC · 114670-114785 · 116 bp

Back to Anacolosa

Pi 0.0561

MarkerSeek score 71.40

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 116
variable_sites: 6
pi: 0.0561
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GTGAAATCGGTAGACACGCT	ACCTGGGATGGTTATTTCGC	50.0	50.0	58.0	57.9	502 bp	0.5	59.5
trnL-UAG-ccsA_p2	TGAAATCGGTAGACACGCTG	ACCTGGGATGGTTATTTCGC	50.0	50.0	58.0	57.9	501 bp	0.5	59.5
trnL-UAG-ccsA_p3	AAGCATCTCGGTTCGAATCC	ACCTGGGATGGTTATTTCGC	50.0	50.0	58.1	57.9	462 bp	0.5	59.1
trnL-UAG-ccsA_p4	GCTAAAGCATCTCGGTTCGA	ACCTGGGATGGTTATTTCGC	50.0	50.0	58.1	57.9	466 bp	0.5	59.0
trnL-UAG-ccsA_p5	TAGACACGCTGCTCTTAGGA	ACCTGGGATGGTTATTTCGC	50.0	50.0	57.9	57.9	492 bp	0.5	58.6