Hotspot

rpl32-trnL-UAG

SSC · 113803-114589 · 787 bp

Back to Anacolosa

Pi 0.0971

MarkerSeek score 80.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 787
variable_sites: 67
pi: 0.0971
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGTCCCCTCCAGTCAAGAAT	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1364-1456 bp	1.0	88.6
rpl32-trnL-UAG_p2	TGTCCCCTCCAGTCAAGAAT	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1365-1457 bp	1.0	88.6
rpl32-trnL-UAG_p3	GCGGAAAAATAGCTCGATGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1278 bp	0.5	59.7
rpl32-trnL-UAG_p4	GCGGAAAAATAGCTCGATGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1279 bp	0.5	59.7
rpl32-trnL-UAG_p5	GCGGAAAAATAGCTCGATGC	ACCTGGGATGGTTATTTCGC	50.0	50.0	58.0	57.9	1760 bp	0.5	59.4