Hotspot

petA-psbJ

LSC · 67537-68477 · 941 bp

Back to Anacolosa

Pi 0.0249

MarkerSeek score 70.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 941
variable_sites: 23
pi: 0.0249
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CATTACGTGTCCAAGGCCTT	CCGGATTGGGTTCATCTCTG	50.0	55.0	58.2	58.0	1080-1232 bp	1.0	81.1
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	TCCGGATTGGGTTCATCTCT	50.0	50.0	58.2	58.1	1081-1233 bp	1.0	80.5
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	TGGTATCCCTGTGATCGGTT	50.0	50.0	58.2	58.1	1130-1282 bp	1.0	80.3
petA-psbJ_p4	TTACGTGTCCAAGGCCTTTT	CCGGATTGGGTTCATCTCTG	45.0	55.0	57.6	58.0	1078-1230 bp	1.0	79.8
petA-psbJ_p5	TACGTGTCCAAGGCCTTTTG	CCGGATTGGGTTCATCTCTG	50.0	55.0	58.4	58.0	1077-1229 bp	1.0	79.4