Hotspot

trnS-GGA-rps4

LSC · 48715-48878 · 164 bp

Back to Anacolosa

Pi 0.0427

MarkerSeek score 70.40

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 164
variable_sites: 7
pi: 0.0427
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	GTGGACTTTTGTTTGCCGAG	TGACTCTTCACCCATTCCAA	50.0	45.0	57.9	56.4	322-437 bp	1.0	67.4
trnS-GGA-rps4_p2	GTGGACTTTTGTTTGCCGAG	TTGACTCTTCACCCATTCCA	50.0	45.0	57.9	56.4	323-438 bp	1.0	67.4
trnS-GGA-rps4_p3	CTTTTGTTTGCCGAGGGTTC	TGACTCTTCACCCATTCCAA	50.0	45.0	57.9	56.4	317-432 bp	1.0	67.3
trnS-GGA-rps4_p4	CTTTTGTTTGCCGAGGGTTC	TTGACTCTTCACCCATTCCA	50.0	45.0	57.9	56.4	318-433 bp	1.0	67.3
trnS-GGA-rps4_p5	GTGGACTTTTGTTTGCCGAG	TTGACTCTTCACCCATTCCAA	50.0	42.9	57.9	57.1	323-438 bp	1.0	65.5