Hotspot

ycf3-trnS-GGA

LSC · 47483-48627 · 1145 bp

Back to Anacolosa

Pi 0.0232
MarkerSeek score 64.60
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
1145
variable_sites
15
pi
0.0232
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.57
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 ATTTATCCGCGATCTAGGCA CTCGGCAAACAAAAGTCCAC 45.0 50.0 56.6 57.9 734-1231 bp 1.0 72.9
ycf3-trnS-GGA_p2 TTTATCCGCGATCTAGGCAT CTCGGCAAACAAAAGTCCAC 45.0 50.0 56.6 57.9 733-1230 bp 1.0 72.9
ycf3-trnS-GGA_p3 ATTTATCCGCGATCTAGGCA GAACCCTCGGCAAACAAAAG 45.0 50.0 56.6 57.9 739-1236 bp 1.0 72.8
ycf3-trnS-GGA_p4 TTTATCCGCGATCTAGGCAT GAACCCTCGGCAAACAAAAG 45.0 50.0 56.6 57.9 738-1235 bp 1.0 72.8
ycf3-trnS-GGA_p5 TTTATCCGCGATCTAGGCAT ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.6 58.2 756-1253 bp 1.0 72.6