Hotspot

trnR-UCU-atpA

LSC · 11036-11244 · 209 bp

Back to Anacolosa

Pi 0.0622

MarkerSeek score 67.90

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 209
variable_sites: 13
pi: 0.0622
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AGCCTTCCAAGCTAACGATG	CGCGGTGGAAGAACAGATAA	50.0	50.0	58.0	58.0	754-775 bp	1.0	85.1
trnR-UCU-atpA_p2	CCTAGCCTTCCAAGCTAACG	CGCGGTGGAAGAACAGATAA	55.0	50.0	58.1	58.0	757-778 bp	1.0	84.8
trnR-UCU-atpA_p3	AGCCTTCCAAGCTAACGATG	ATTGGAACTGGCGCAATTTG	50.0	45.0	58.0	57.9	889-910 bp	1.0	83.6
trnR-UCU-atpA_p4	AGCCTTCCAAGCTAACGATG	GGTCGTGAAGCTTATCCAGG	50.0	55.0	58.0	58.1	1194-1215 bp	1.0	82.9
trnR-UCU-atpA_p5	CCTAGCCTTCCAAGCTAACG	GGTCGTGAAGCTTATCCAGG	55.0	55.0	58.1	58.1	1197-1218 bp	1.0	82.7