Hotspot

psbI-trnS-GCU

LSC · 8995-9135 · 141 bp

Back to Anacolosa

Pi 0.0615

MarkerSeek score 77.40

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 141
variable_sites: 8
pi: 0.0615
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGTAATCCTGGACGTGAAGA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.1	58.0	227-233 bp	1.0	78.8
psbI-trnS-GCU_p2	CGTAATCCTGGACGTGAAGAA	TAAAGCGGCGGATTGCTAAT	47.6	45.0	57.8	58.0	227-233 bp	1.0	76.5
psbI-trnS-GCU_p3	CGTAATCCTGGACGTGAAGA	CGAGTTATTCGTACCGAGGG	50.0	55.0	57.1	57.6	199-205 bp	1.0	76.5
psbI-trnS-GCU_p4	CGTAATCCTGGACGTGAAGA	ATGGCTGAGTGGACTAAAGC	50.0	50.0	57.1	57.6	241-247 bp	1.0	75.5
psbI-trnS-GCU_p5	CCTGTCTAATGATCCAGGACG	TAAAGCGGCGGATTGCTAAT	52.4	45.0	57.6	58.0	246-252 bp	1.0	74.8