Hotspot

rpl22

LSC · 83326-83940 · 615 bp

Back to Anabasis

Pi 0.021

MarkerSeek score 62.80

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 615
variable_sites: 30
pi: 0.021
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TGTGCCAAGTCTGAAACCAA	AAGAAATATGGATCCTTAACATTT	45.0	25.0	57.9	52.4	706 bp	1.0	43.4
rpl22_p2	TTGTGCCAAGTCTGAAACCA	AAGAAATATGGATCCTTAACATTT	45.0	25.0	57.9	52.4	707 bp	1.0	43.4
rpl22_p3	TGCCAAGTCTGAAACCAAGT	AAGAAATATGGATCCTTAACATTT	45.0	25.0	57.6	52.4	704 bp	1.0	43.4
rpl22_p4	AATGACTTTGGGTTGTGCCA	AAGAAATATGGATCCTTAACATTT	45.0	25.0	58.2	52.4	719 bp	1.0	43.3
rpl22_p5	ATGACTTTGGGTTGTGCCAA	AAGAAATATGGATCCTTAACATTT	45.0	25.0	58.2	52.4	718 bp	1.0	43.3