Hotspot

trnL-CAA-ycf15

IRa · 142176-142751 · 576 bp

Back to Anabasis

Pi 0.00463

MarkerSeek score 63.90

Primer available yes

Label trnL-CAA-ycf15

Feature Metadata

label_name: trnL-CAA-ycf15
feature_type: igs
region: IRa
length_bp: 576
variable_sites: 8
pi: 0.00463
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf15_p1	AAATGGTAGACACGCGAGAC	TCTTGAACAGCGAACAACCA	50.0	45.0	58.0	58.0	752-764 bp	1.0	81.5
trnL-CAA-ycf15_p2	AAATGGTAGACACGCGAGAC	TTGAACAGCGAACAACCAGA	50.0	45.0	58.0	58.0	750-762 bp	1.0	81.5
trnL-CAA-ycf15_p3	TGAAATGGTAGACACGCGAG	TCTTGAACAGCGAACAACCA	50.0	45.0	58.0	58.0	754-766 bp	1.0	81.5
trnL-CAA-ycf15_p4	TGAAATGGTAGACACGCGAG	TTGAACAGCGAACAACCAGA	50.0	45.0	58.0	58.0	752-764 bp	1.0	81.5
trnL-CAA-ycf15_p5	GCCTTGGTGGTGAAATGGTA	TTGAACAGCGAACAACCAGA	50.0	45.0	58.1	58.0	762-774 bp	1.0	80.9