Hotspot

ccsA-ndhD

SSC · 113577-113769 · 193 bp

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Pi 0.0311

MarkerSeek score 66.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 193
variable_sites: 14
pi: 0.0311
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATGCAGCTCTTTTATGCGGA	TTTTTGATTCTGGGCCACGA	45.0	45.0	58.0	58.0	863-873 bp	1.0	83.5
ccsA-ndhD_p2	TGCAGCTCTTTTATGCGGAT	TTTTTGATTCTGGGCCACGA	45.0	45.0	58.0	58.0	862-872 bp	1.0	83.5
ccsA-ndhD_p3	ATGCAGCTCTTTTATGCGGA	AAATGGGCGGAATAGCGATT	45.0	45.0	58.0	58.0	1134-1146 bp	1.0	83.1
ccsA-ndhD_p4	TGCAGCTCTTTTATGCGGAT	AAATGGGCGGAATAGCGATT	45.0	45.0	58.0	58.0	1133-1145 bp	1.0	83.1
ccsA-ndhD_p5	GCTACGTCAGGCCTCTTAAC	TTTTTGATTCTGGGCCACGA	55.0	45.0	58.1	58.0	966-976 bp	1.0	82.8