Hotspot

rpl32-trnL-UAG

SSC · 111441-112380 · 940 bp

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Pi 0.0162

MarkerSeek score 64.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 940
variable_sites: 38
pi: 0.0162
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGCAGCGTTAAAAGCCTTTT	ATCCGCATAAAAGAGCTGCA	45.0	45.0	57.9	58.0	1646-1717 bp	1.0	85.2
rpl32-trnL-UAG_p2	GGCAGCGTTAAAAGCCTTTT	TCCGCATAAAAGAGCTGCAT	45.0	45.0	57.9	58.0	1645-1716 bp	1.0	85.2
rpl32-trnL-UAG_p3	GGCAGCGTTAAAAGCCTTTT	GTTAAGAGGCCTGACGTAGC	45.0	55.0	57.9	58.1	1542-1613 bp	1.0	85.1
rpl32-trnL-UAG_p4	CCTTTCGACCGGGAATTCAA	ATCCGCATAAAAGAGCTGCA	50.0	45.0	58.2	58.0	1613-1677 bp	0.666667	70.7
rpl32-trnL-UAG_p5	CCTTTCGACCGGGAATTCAA	TCCGCATAAAAGAGCTGCAT	50.0	45.0	58.2	58.0	1612-1676 bp	0.666667	70.7