Hotspot

trnN-GUU-ndhF

IRb · 107263-107943 · 681 bp

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Pi 0.0278

MarkerSeek score 73.30

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 681
variable_sites: 42
pi: 0.0278
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGGATTCGAACCTACGACCA	GAGTCGAATCTCCTCCTATCT	50.0	47.6	58.2	55.5	659-1665 bp	1.0	57.3
trnN-GUU-ndhF_p2	AATCAGTTAACAGCCGACCG	GAGTCGAATCTCCTCCTATCT	50.0	47.6	58.3	55.5	640-1646 bp	1.0	56.4
trnN-GUU-ndhF_p3	CCCCAAGTAGGATTCGAACC	GAGTCGAATCTCCTCCTATCT	55.0	47.6	57.7	55.5	667-1673 bp	1.0	55.9
trnN-GUU-ndhF_p4	GCGAGTCCCTTCATTCTGAA	GAGTCGAATCTCCTCCTATCT	50.0	47.6	57.6	55.5	714-1720 bp	1.0	55.4
trnN-GUU-ndhF_p5	AAGCGAGTCCCTTCATTCTG	GAGTCGAATCTCCTCCTATCT	50.0	47.6	57.6	55.5	716-1722 bp	1.0	55.3