Hotspot

ycf15-trnL-CAA

IRb · 92320-92895 · 576 bp

Back to Anabasis

Pi 0.00463

MarkerSeek score 63.90

Primer available yes

Label ycf15-trnL-CAA

Feature Metadata

label_name: ycf15-trnL-CAA
feature_type: igs
region: IRb
length_bp: 576
variable_sites: 8
pi: 0.00463
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-trnL-CAA_p1	TCTTGAACAGCGAACAACCA	AAATGGTAGACACGCGAGAC	45.0	50.0	58.0	58.0	752-764 bp	1.0	81.5
ycf15-trnL-CAA_p2	TTGAACAGCGAACAACCAGA	AAATGGTAGACACGCGAGAC	45.0	50.0	58.0	58.0	750-762 bp	1.0	81.5
ycf15-trnL-CAA_p3	TCTTGAACAGCGAACAACCA	TGAAATGGTAGACACGCGAG	45.0	50.0	58.0	58.0	754-766 bp	1.0	81.5
ycf15-trnL-CAA_p4	TTGAACAGCGAACAACCAGA	TGAAATGGTAGACACGCGAG	45.0	50.0	58.0	58.0	752-764 bp	1.0	81.5
ycf15-trnL-CAA_p5	TTGAACAGCGAACAACCAGA	GCCTTGGTGGTGAAATGGTA	45.0	50.0	58.0	58.1	762-774 bp	1.0	80.9