Hotspot

trnF-GAA-ndhJ

LSC · 48285-48971 · 687 bp

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Pi 0.0174

MarkerSeek score 69.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 687
variable_sites: 29
pi: 0.0174
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	779-819 bp	1.0	85.1
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TCCACGCCTGAAACGTATTT	50.0	45.0	58.1	57.8	810-850 bp	1.0	84.2
trnF-GAA-ndhJ_p3	AAGGGTGAATGAATGGTCGG	AGTTGGATAGGATGGCCCTT	50.0	50.0	57.9	58.1	833-873 bp	1.0	84.1
trnF-GAA-ndhJ_p4	AATGGTCGGGATAGCTCAGT	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.2	58.1	822-862 bp	1.0	83.6
trnF-GAA-ndhJ_p5	ATGGTCGGGATAGCTCAGTT	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.2	58.1	821-861 bp	1.0	83.6