Hotspot

trnL-UAA-trnF-GAA

LSC · 47836-48211 · 376 bp

Back to Anabasis

Pi 0.0326

MarkerSeek score 63.90

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 376
variable_sites: 28
pi: 0.0326
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	464-471 bp	1.0	83.1
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	58.1	479-486 bp	1.0	82.6
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	CATGTGCCAGGAACCAGATT	50.0	50.0	57.5	58.2	482-489 bp	1.0	82.5
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGA	50.0	50.0	57.5	57.6	426-433 bp	1.0	80.6
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	TGTGCCAGGAACCAGATTTG	50.0	50.0	57.5	58.4	480-487 bp	1.0	80.4