Hotspot

psaA-ycf3

LSC · 41613-42375 · 763 bp

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Pi 0.0171

MarkerSeek score 64.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 763
variable_sites: 32
pi: 0.0171
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	AGTTTCAGGGCCCTTAGCTA	CGGAAATTGCAGAAGCTTGG	50.0	50.0	58.0	58.0	978-1013 bp	1.0	86.2
psaA-ycf3_p2	TAGTTTCAGGGCCCTTAGCT	CGGAAATTGCAGAAGCTTGG	50.0	50.0	58.0	58.0	979-1014 bp	1.0	86.2
psaA-ycf3_p3	GTAGTTTCAGGGCCCTTAGC	CGGAAATTGCAGAAGCTTGG	55.0	50.0	58.0	58.0	980-1015 bp	1.0	86.1
psaA-ycf3_p4	GGCACTAGGTCCAATGTGAG	CGGAAATTGCAGAAGCTTGG	55.0	50.0	58.0	58.0	1188-1223 bp	1.0	85.1
psaA-ycf3_p5	GTTATTCCAGATGCTCGCCA	CGGAAATTGCAGAAGCTTGG	50.0	50.0	58.0	58.0	1304-1339 bp	1.0	84.5