Hotspot

trnG-GCC-trnR-UCU

LSC · 9574-9688 · 115 bp

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Pi 0.0267

MarkerSeek score 64.20

Primer available yes

Label trnG-GCC-trnR-UCU

Feature Metadata

label_name: trnG-GCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 115
variable_sites: 8
pi: 0.0267
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	CCCCTCTCACGGTAGAAGAA	50.0	55.0	58.0	58.2	569-577 bp	1.0	82.6
trnG-GCC-trnR-UCU_p2	AGCCTTCCAAGCTAACGATG	GACCAGCGATTAATGTGGGT	50.0	50.0	58.0	58.0	785-793 bp	1.0	82.4
trnG-GCC-trnR-UCU_p3	CCTAGCCTTCCAAGCTAACG	GACCAGCGATTAATGTGGGT	55.0	50.0	58.1	58.0	788-796 bp	1.0	82.2
trnG-GCC-trnR-UCU_p4	AGCCTTCCAAGCTAACGATG	TTGGCGCAATTTGCAGAATT	50.0	40.0	58.0	57.8	690-698 bp	1.0	81.8
trnG-GCC-trnR-UCU_p5	TCGTTCCCACCTTCGATTTC	GACCAGCGATTAATGTGGGT	50.0	50.0	57.9	58.0	923-931 bp	1.0	81.7