Hotspot

trnS-GCU-trnG-GCC

LSC · 7964-8776 · 813 bp

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Pi 0.0341

MarkerSeek score 73.00

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 813
variable_sites: 51
pi: 0.0341
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CGAATGACTCGTACAACGGA	ACGAATCACACTTTTACCACT	50.0	38.1	57.7	55.5	813-906 bp	1.0	58.4
trnS-GCU-trnG-GCC_p2	CGAATGACTCGTACAACGGA	AGAACGAATCACACTTTTACCAC	50.0	39.1	57.7	57.3	816-909 bp	1.0	57.4
trnS-GCU-trnG-GCC_p3	CGAATGACTCGTACAACGGA	GAACGAATCACACTTTTACCACT	50.0	39.1	57.7	57.3	815-908 bp	1.0	57.4
trnS-GCU-trnG-GCC_p4	CGAATGACTCGTACAACGGA	AACGAATCACACTTTTACCACT	50.0	36.4	57.7	56.2	814-907 bp	1.0	56.5
trnS-GCU-trnG-GCC_p5	CTTTCGTCCACTCAGCCATC	ACGAATCACACTTTTACCACT	55.0	38.1	58.6	55.5	779-872 bp	1.0	55.6