Hotspot

rps16-trnQ-UUG

LSC · 6039-6575 · 537 bp

Back to Anabasis

Pi 0.0166

MarkerSeek score 68.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 537
variable_sites: 22
pi: 0.0166
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	427-629 bp	1.0	85.3
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	438-640 bp	1.0	84.7
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	45.0	50.0	58.1	58.2	457-659 bp	1.0	84.4
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	TAAGGCATCGGGTTTTGGTC	50.0	50.0	58.2	58.2	468-670 bp	1.0	83.8
rps16-trnQ-UUG_p5	TGTCGAGTCAAGAGCATCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	495-697 bp	1.0	82.5