Hotspot

trnH-GUG-psbA

LSC · 75-307 · 233 bp

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Pi 0.03

MarkerSeek score 64.90

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 233
variable_sites: 13
pi: 0.03
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	ATTTCAGGCTGAGCACAACA	55.0	45.0	58.1	58.0	756-766 bp	1.0	82.4
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	TTTCAGGCTGAGCACAACAT	55.0	45.0	58.1	58.0	755-765 bp	1.0	82.4
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	ATTTCAGGCTGAGCACAACA	50.0	45.0	57.9	58.0	770-780 bp	1.0	82.3
trnH-GUG-psbA_p4	CAATCCACTGCCTTGATCCA	TTTCAGGCTGAGCACAACAT	50.0	45.0	57.9	58.0	769-779 bp	1.0	82.3
trnH-GUG-psbA_p5	AATCCACTGCCTTGATCCAC	TTTCAGGCTGAGCACAACAT	50.0	45.0	57.9	58.0	768-778 bp	1.0	82.3