Hotspot

psaC-ycf1

SSC · 95304-95727 · 424 bp

Back to Amyema

Pi 0.0998
MarkerSeek score 78.00
Primer available yes
Label psaC-ycf1

Feature Metadata

label_name
psaC-ycf1
feature_type
igs
region
SSC
length_bp
424
variable_sites
81
pi
0.0998
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ycf1_p1 CACATTGAGTGCACCCGATA TGAACCGATTCGTCTGTCTG 50.0 50.0 58.0 57.7 825-948 bp 1.0 89.0
psaC-ycf1_p2 CATTGAGTGCACCCGATACA TGAACCGATTCGTCTGTCTG 50.0 50.0 58.0 57.7 823-946 bp 1.0 89.0
psaC-ycf1_p3 TTGAGTGCACCCGATACATG TGAACCGATTCGTCTGTCTG 50.0 50.0 58.0 57.7 821-944 bp 1.0 89.0
psaC-ycf1_p4 ACATTGAGTGCACCCGATAC TGAACCGATTCGTCTGTCTG 50.0 50.0 58.0 57.7 824-947 bp 1.0 89.0
psaC-ycf1_p5 TTGAGTGCACCCGATACATG GAACCGATTCGTCTGTCTGT 50.0 50.0 58.0 57.7 820-943 bp 1.0 89.0