Hotspot

trnL-CUA-ccsA

SSC · 93659-93756 · 98 bp

Back to Amyema

Pi 0.0969
MarkerSeek score 79.30
Primer available yes
Label trnL-CUA-ccsA

Feature Metadata

label_name
trnL-CUA-ccsA
feature_type
igs
region
SSC
length_bp
98
variable_sites
19
pi
0.0969
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CUA-ccsA_p1 ACGCTGTTCTTAGGAAGCAG ATCCGCATAAAAGAGCTGCA 50.0 45.0 57.9 58.0 609-628 bp 1.0 87.2
trnL-CUA-ccsA_p2 ACGCTGTTCTTAGGAAGCAG TCCGCATAAAAGAGCTGCAT 50.0 45.0 57.9 58.0 608-627 bp 1.0 87.2
trnL-CUA-ccsA_p3 CGCTGTTCTTAGGAAGCAGT ATCCGCATAAAAGAGCTGCA 50.0 45.0 57.9 58.0 608-627 bp 1.0 87.2
trnL-CUA-ccsA_p4 CGCTGTTCTTAGGAAGCAGT TCCGCATAAAAGAGCTGCAT 50.0 45.0 57.9 58.0 607-626 bp 1.0 87.2
trnL-CUA-ccsA_p5 CCGAGAAGAAGGTATCAGCG TCCGCATAAAAGAGCTGCAT 55.0 45.0 57.9 58.0 1277-1299 bp 0.8 73.0