Hotspot

trnL-UUG-rps7

IRb · 81026-82230 · 1205 bp

Back to Amyema

Pi 0.0536
MarkerSeek score 84.70
Primer available yes
Label trnL-UUG-rps7

Feature Metadata

label_name
trnL-UUG-rps7
feature_type
igs
region
IRb
length_bp
1205
variable_sites
108
pi
0.0536
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UUG-rps7_p1 GTCTCGCGTGTCTACCATTT ATGGCAGAGGCAAATAGAGC 50.0 50.0 58.0 58.0 568-1276 bp 1.0 91.6
trnL-UUG-rps7_p2 CTCGCGTGTCTACCATTTCA ATGGCAGAGGCAAATAGAGC 50.0 50.0 58.0 58.0 566-1274 bp 1.0 91.6
trnL-UUG-rps7_p3 GTCTCGCGTGTCTACCATTT GGCAGAGGCAAATAGAGCTT 50.0 50.0 58.0 58.0 566-1274 bp 1.0 91.4
trnL-UUG-rps7_p4 CTCGCGTGTCTACCATTTCA GGCAGAGGCAAATAGAGCTT 50.0 50.0 58.0 58.0 564-1272 bp 1.0 91.4
trnL-UUG-rps7_p5 TACCATTTCACCACCAAGGC ATGGCAGAGGCAAATAGAGC 50.0 50.0 58.1 58.0 556-1264 bp 1.0 91.1