Hotspot

trnS-UCA-psbZ

LSC · 29555-29913 · 359 bp

Back to Amyema

Pi 0.0573
MarkerSeek score 83.70
Primer available yes
Label trnS-UCA-psbZ

Feature Metadata

label_name
trnS-UCA-psbZ
feature_type
igs
region
LSC
length_bp
359
variable_sites
42
pi
0.0573
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UCA-psbZ_p1 CAACCACTCAGCCATCTCTC GCAAAAACAGCTAATTGGAAAGC 55.0 39.1 58.0 58.0 412-677 bp 1.0 66.3
trnS-UCA-psbZ_p2 GAGCTATCAACCACTCAGCC GCAAAAACAGCTAATTGGAAAGC 55.0 39.1 58.1 58.0 419-684 bp 1.0 65.9
trnS-UCA-psbZ_p3 GGAGCTATCAACCACTCAGC GCAAAAACAGCTAATTGGAAAGC 55.0 39.1 58.1 58.0 420-685 bp 1.0 65.9
trnS-UCA-psbZ_p4 CAAGACCGGAGCTATCAACC GCAAAAACAGCTAATTGGAAAGC 55.0 39.1 58.1 58.0 427-692 bp 1.0 65.8
trnS-UCA-psbZ_p5 CGGAGCTATCAACCACTCAG GCAAAAACAGCTAATTGGAAAGC 55.0 39.1 57.8 58.0 421-686 bp 1.0 64.6