Hotspot

trnC-UGC-petN

LSC · 22446-22731 · 286 bp

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Pi 0.0951
MarkerSeek score 83.40
Primer available yes
Label trnC-UGC-petN

Feature Metadata

label_name
trnC-UGC-petN
feature_type
igs
region
LSC
length_bp
286
variable_sites
49
pi
0.0951
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-UGC-petN_p1 TTTCCCCAGTTCAAATCCGG ATTAAAGCAGCCCAAGCGAT 50.0 45.0 58.1 58.2 300-396 bp 1.0 89.5
trnC-UGC-petN_p2 GTATCATTTTGGCGGCATGG ATTAAAGCAGCCCAAGCGAT 50.0 45.0 57.9 58.2 352-448 bp 1.0 89.0
trnC-UGC-petN_p3 TTTCCCCAGTTCAAATCCGG TAAAGCAGCCCAAGCGATAC 50.0 50.0 58.1 58.3 298-394 bp 1.0 88.7
trnC-UGC-petN_p4 GTATCATTTTGGCGGCATGG TAAAGCAGCCCAAGCGATAC 50.0 50.0 57.9 58.3 350-446 bp 1.0 88.2
trnC-UGC-petN_p5 TTTCCCCAGTTCAAATCCGG TTAAAGCAGCCCAAGCGATA 50.0 45.0 58.1 57.6 299-395 bp 1.0 88.0