Hotspot

ycf1

SSC · 124083-129593 · 5511 bp

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Pi 0.00913

MarkerSeek score 45.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5511
variable_sites: 50
pi: 0.00913
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTTGTGTATTGAGTCTGGGA	AAAATAACCCCACCAGGAGT	42.9	45.0	56.3	56.3	218 bp	1.0	47.0
ycf1_p2	AAGTTGTGTATTGAGTCTGGGA	AAAATAACCCCACCAGGAGT	40.9	45.0	57.0	56.3	219 bp	1.0	44.8
ycf1_p3	AGTTGTGTATTGAGTCTGGGAA	AAAATAACCCCACCAGGAGT	40.9	45.0	57.0	56.3	218 bp	1.0	44.8
ycf1_p4	AGCCTTTCTTGATTTGCTTCA	AAAATAACCCCACCAGGAGT	38.1	45.0	56.3	56.3	272 bp	0.5	25.1
ycf1_p5	AAGCCTTTCTTGATTTGCTTCA	AAAATAACCCCACCAGGAGT	36.4	45.0	57.1	56.3	273 bp	0.5	22.9