Hotspot

ndhF

SSC · 111097-113331 · 2235 bp

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Pi 0.00984

MarkerSeek score 56.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2235
variable_sites: 22
pi: 0.00984
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGCCCCGTTTATGAGGATTC	GAGGTAAGATCCGGTCAATCG	50.0	52.4	57.4	58.0	2393-2440 bp	1.0	67.5
ndhF_p2	GCCCCGTTTATGAGGATTCT	GAGGTAAGATCCGGTCAATCG	50.0	52.4	57.4	58.0	2392-2439 bp	1.0	67.5
ndhF_p3	AGCCCCGTTTATGAGGATTC	AGGTAAGATCCGGTCAATCG	50.0	50.0	57.4	56.8	2392-2439 bp	1.0	66.2
ndhF_p4	GCCCCGTTTATGAGGATTCT	AGGTAAGATCCGGTCAATCG	50.0	50.0	57.4	56.8	2391-2438 bp	1.0	66.2
ndhF_p5	AAAAGCCCCGTTTATGAGGA	GAGGTAAGATCCGGTCAATCG	45.0	52.4	57.1	58.0	2396-2443 bp	1.0	65.5