Hotspot

ndhG-ndhI

SSC · 119164-119396 · 233 bp

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Pi 0.0402

MarkerSeek score 71.70

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 233
variable_sites: 9
pi: 0.0402
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ACCTCCTAAGATCAGACCCG	TCAAATTGCTTTGGGTCGGT	55.0	45.0	57.9	58.3	402-637 bp	1.0	79.8
ndhG-ndhI_p2	ATCAGACCCGATCCCAGAAA	TCAAATTGCTTTGGGTCGGT	50.0	45.0	58.1	58.3	392-627 bp	1.0	79.5
ndhG-ndhI_p3	ACCTCCTAAGATCAGACCCG	TCGAATTCGCCTCAAACTCA	55.0	45.0	57.9	57.6	341-576 bp	1.0	79.0
ndhG-ndhI_p4	ATCAGACCCGATCCCAGAAA	TCGAATTCGCCTCAAACTCA	50.0	45.0	58.1	57.6	331-566 bp	1.0	78.7
ndhG-ndhI_p5	ACCTCCTAAGATCAGACCCG	CAAATTGCTTTGGGTCGGTT	55.0	45.0	57.9	57.5	401-636 bp	1.0	78.0