Hotspot

ndhE-ndhG

SSC · 118529-118632 · 104 bp

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Pi 0.0192

MarkerSeek score 63.60

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 104
variable_sites: 2
pi: 0.0192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TTCGGCTTGTGATCAATCCA	AGGTGCAATTGCTGTAGCTC	45.0	50.0	57.5	58.5	208 bp	1.0	72.5
ndhE-ndhG_p2	TCGGCTTGTGATCAATCCAT	AGGTGCAATTGCTGTAGCTC	45.0	50.0	57.3	58.5	207 bp	1.0	70.7
ndhE-ndhG_p3	TTCGGCTTGTGATCAATCCA	GCAATTGCTGTAGCTCGTCA	45.0	50.0	57.5	58.9	204 bp	1.0	69.5
ndhE-ndhG_p4	TTCGGCTTGTGATCAATCCA	TGCAATTGCTGTAGCTCGTC	45.0	50.0	57.5	58.9	205 bp	1.0	69.5
ndhE-ndhG_p5	TTCGGCTTGTGATCAATCCAT	AGGTGCAATTGCTGTAGCTC	42.9	50.0	58.0	58.5	208 bp	1.0	68.4