Hotspot

cemA-petA

LSC · 62947-63203 · 257 bp

Back to Amsonia

Pi 0.0195

MarkerSeek score 63.70

Primer available yes

Label cemA-petA

Feature Metadata

label_name: cemA-petA
feature_type: igs
region: LSC
length_bp: 257
variable_sites: 5
pi: 0.0195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-petA_p1	CGTGTATCTCCGTCACTTGT	GAAATGGCCGTCCGAGTTAT	50.0	50.0	57.4	58.1	406 bp	1.0	75.5
cemA-petA_p2	CGTGTATCTCCGTCACTTGT	ATGCATTTGAAATGGCCGTC	50.0	45.0	57.4	57.7	414 bp	1.0	73.5
cemA-petA_p3	CGTGTATCTCCGTCACTTGT	TGGCCGTCCGAGTTATGATA	50.0	50.0	57.4	58.3	402 bp	1.0	73.5
cemA-petA_p4	TCGTGTATCTCCGTCACTTG	GAAATGGCCGTCCGAGTTAT	50.0	50.0	57.1	58.1	407 bp	1.0	73.3
cemA-petA_p5	CGTGTATCTCCGTCACTTGT	AAATGGCCGTCCGAGTTATG	50.0	50.0	57.4	58.3	405 bp	1.0	73.2