Hotspot

psaI-ycf4

LSC · 60818-61143 · 326 bp

Back to Amsonia

Pi 0.0276

MarkerSeek score 54.30

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 326
variable_sites: 9
pi: 0.0276
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GCAATCGCAATGGCTTCTTT	ATTGCCCACAGCCAACAATA	45.0	45.0	58.0	58.1	1125-1354 bp	1.0	82.3
psaI-ycf4_p2	GCAATCGCAATGGCTTCTTT	ACACGCAAGAAATAGGCCAA	45.0	45.0	58.0	58.1	907-1021 bp	1.0	81.4
psaI-ycf4_p3	GCAATCGCAATGGCTTCTTT	AATCGGAGGAATATGCGACG	45.0	50.0	58.0	57.9	739-844 bp	1.0	81.3
psaI-ycf4_p4	GCAATCGCAATGGCTTCTTT	CGGAGGAATATGCGACGATT	45.0	50.0	58.0	57.9	736-841 bp	1.0	81.3
psaI-ycf4_p5	GCAATCGCAATGGCTTCTTT	CCAGGAAATCCCCAACGAAA	45.0	50.0	58.0	58.1	712-817 bp	1.0	81.2