Hotspot

trnT-GGU-psbD

LSC · 32250-33534 · 1285 bp

Back to Amsonia

Pi 0.00948

MarkerSeek score 65.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1285
variable_sites: 12
pi: 0.00948
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.1	58.0	1419-1427 bp	1.0	81.2
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1422-1430 bp	1.0	81.0
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1437-1445 bp	1.0	80.3
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1440-1448 bp	1.0	80.1
trnT-GGU-psbD_p5	ATAGTGACCCGACCCATTCA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1478 bp	0.5	59.0