Hotspot

trnD-GUC-trnY-GUA

LSC · 31011-31108 · 98 bp

Back to Amsonia

Pi 0.0306
MarkerSeek score 65.50
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
98
variable_sites
3
pi
0.0306
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 CGGTGCTCTGACCAATTGAA TGGCAATATGTCTACGCTGG 50.0 50.0 58.5 57.8 168 bp 1.0 75.7
trnD-GUC-trnY-GUA_p2 CGGTGCTCTGACCAATTGAA GGCAATATGTCTACGCTGGT 50.0 50.0 58.5 57.8 167 bp 1.0 75.7
trnD-GUC-trnY-GUA_p3 GGCCCCCTTTCGAATTGATT TGGCAATATGTCTACGCTGG 50.0 50.0 58.5 57.8 252 bp 1.0 74.9
trnD-GUC-trnY-GUA_p4 GGCCCCCTTTCGAATTGATT GGCAATATGTCTACGCTGGT 50.0 50.0 58.5 57.8 251 bp 1.0 74.9
trnD-GUC-trnY-GUA_p5 GCCCCCTTTCGAATTGATTG TGGCAATATGTCTACGCTGG 50.0 50.0 57.4 57.8 251 bp 1.0 74.4